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Dilution Formula:
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A 1:1000 dilution means one part of the original solution is mixed with 999 parts of diluent (solvent), resulting in a total of 1000 parts. This reduces the concentration of the original solution by a factor of 1000.
The calculator uses the dilution formula:
Where:
For 1:1000 dilution:
Details: Accurate dilutions are essential in laboratory work, medicine preparation, and various scientific applications to achieve desired concentrations for experiments or treatments.
Tips: Enter the initial concentration (mg/mL) and initial volume (mL) of your solution. The calculator will determine the final concentration, final volume, and amount of solvent needed for a 1:1000 dilution.
Q1: When would I use a 1:1000 dilution?
A: This high dilution factor is often used when working with very concentrated stock solutions or when preparing extremely dilute solutions for sensitive assays.
Q2: Can I do a 1:1000 dilution in one step?
A: While possible, it's often more accurate to perform serial dilutions (e.g., three 1:10 dilutions) to achieve 1:1000.
Q3: What solvent should I use?
A: Use an appropriate solvent that is compatible with your solute - typically water, buffer solution, or another solvent specified in your protocol.
Q4: How precise does the measurement need to be?
A: Precision requirements depend on the application. For critical applications, use calibrated glassware and precise measuring equipment.
Q5: What if my final volume is too large?
A: Consider using a smaller initial volume or performing serial dilutions to achieve the desired concentration with more manageable volumes.